How can I solve this my abnormal real-time PCR result?

I run a qPCR (Taqman probe) to detect shiga-toxin gene (stx1) with rotar gene Q (qiagen) thermal cycler. My primer & probe are - F - 5'-TTTGTYACTGTSACAGCWGAAGCYTTACG-3' Base - 29, GC - 44.8%,...

18 December 2022 2,950 3 View