Ht Liang

5 Questions 2 Answers 0 Followers

Questions related from Ht Liang

How do I calculate the number of moles of PCL-PEG-PCL triblock copolymer?

Hi all, I am carrying out a catalytic reaction to break down a polymer (PCL-PEG-PCL, 2k-2k-2k). I am unsure how to calculate the number of moles of polymer. Say that I want 3.0 mg of substrate. If...

16 March 2021 1,050 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

03 March 2021 6,476 3 View

When do we use the "Dissolution" Cycle on the autoclave?

I am writing up the standard operating procedure for the autoclave and realised that there is a cycle called "Dissolution". I have not used it before but from the manual that I read, it is used...

11 October 2016 5,126 2 View

Hi all, for a ligation reaction, is it true that incubating the reactions overnight at 4 deg Celsius would yield the maximum number of transformants?

Read this in a protocol for pGEM-T Easy Vector Systems. Normally, we would incubate a ligation reaction for an hour at room temperature, so I am curious if there is an explanation behind...

21 September 2016 1,617 8 View

Is it difficult to ligate a small DNA part (about 30bp long) to a vector? What possible steps could be taken to increase the ligation efficiency?

Carried out ligation a couple of times in different ratios and incubation times. Transformation results: negative has more colonies than positive. Really appreciate the help, thanks.

04 October 2015 8,304 4 View