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Questions related from Hsin-Che Caleb Lin
Hi , I'm trying to scale up, but the only rotor I found in the facility is way too large (which fits 50 mL ultracentrifugal tubes, and I already had these)for my maximal yield (which is 15-20...
03 March 2018 1,008 3 View
Hi, there I am wondering why the result of intact IgG in the sds-page is ca. 200-250 kDa? This is a Mouse IgG2b, κ. Shouldn't the MW of a mouse be around 160 kDa? Now I suspect there might be...
05 May 2017 1,616 6 View
Hi, I am intent to run Tris/Glycine SDS-PAGE for small protein molecules (MW= ca. 6~70 kDa). To prevent the small molecules dissolved in liquid, I performed fixation process by 5% glutaraldehyde...
03 March 2017 9,926 5 View
Usually the mAb products may be categorized by species reactivity, and host according to experiment design. However, how do I know if the mAb product is chimeric, humanzied, or full human? And...
01 January 2017 3,469 4 View