Hosam Elbaz

4 Questions 4 Answers 0 Followers

Questions related from Hosam Elbaz

How stable are immunofluorescence samples in the dark at 4°C?

A month ago, I stained panc-1 cells with Alexa flour 488 and evaluated my samples for adequate staining. I have kept the samples in an opaque box at 4°C since then. I need to re-evaluate my...

15 January 2013 610 33 View

Should immunoblots be reprobed with a single antibody at a time? Or would antibody cocktails do as fine?

Western blotting is usually time consuming. Sometimes, there are a number of proteins that need to be characterized per blot for an experimental run. However, sometimes these cocktails yield low...

19 December 2012 6,510 3 View

How stable are phospho-proteins in western blot samples?

I have been looking into the literature and different protocols for some answers pertaing to the stability of western blot phospho-protein samples in lysis buffer, in loading buffer, and after...

17 December 2012 8,751 16 View

What are sources of inconsistencies in western blot analysis?

I am currently looking at dose dependent effects on protein expression using western blot analysis. So far, I have been getting inconsistent trends in expression and a significant degree of...

24 October 2012 2,022 4 View