Homa Hemmati

3 Questions 13 Answers 0 Followers

Questions related from Homa Hemmati

How can I solve the problem in cloning a PCR amplified gene into a vector?

I am trying to clone two genes on one single vector. One gene is 1800 bp and the other 2900 bp. My vector is 10 kb ( pBIN Vector) and doesn't have my desired restriction sites so I designed...

07 September 2014 991 20 View

How can I find out if my protein is hydrophilic or hydrophobic from the sequence?

I am working on a novel gene which supposed to be present in the apoplast. I am trying to figure out to see if the protein is hyrophilic. I have tried a couple of website online but they give me a...

05 May 2014 4,809 5 View

Why did I get band for RNA after PCR?

I did cDNA synthesis after RNA extraction including a negative control containing RNA and water with no RT. I used all my samples for regular PCR While I included a negative control containing...

28 February 2014 8,890 14 View