Homa Hemmati

4 Questions 15 Answers 0 Followers

Questions related from Homa Hemmati

How can I solve the problem in cloning a PCR amplified gene into a vector?

I am trying to clone two genes on one single vector. One gene is 1800 bp and the other 2900 bp. My vector is 10 kb ( pBIN Vector) and doesn't have my desired restriction sites so I designed...

07 September 2014 1,054 20 View

How can I find out if my protein is hydrophilic or hydrophobic from the sequence?

I am working on a novel gene which supposed to be present in the apoplast. I am trying to figure out to see if the protein is hyrophilic. I have tried a couple of website online but they give me a...

05 May 2014 4,850 5 View

Why did I get band for RNA after PCR?

I did cDNA synthesis after RNA extraction including a negative control containing RNA and water with no RT. I used all my samples for regular PCR While I included a negative control containing...

28 February 2014 8,977 14 View

Can anyone suggest a fast and efficient method to transfer a gene to Arabidopsis and tobacco plants?

I've been doing tobacco leaf transformation and floral dip for Arabidopsis many times for over a year. Unfortunately I haven't got my desired results. For leaf transformation I have got no...

28 January 2014 2,573 11 View