Homa Ahmadi

11 Questions 30 Answers 0 Followers

Questions related from Homa Ahmadi

How cell wall peroperties of l.lactis may change after surface display of a heterogenous antigen?

Hi there! We are applying L.lactis for expression of a heterogeneous antigen using a cell wall anchor subunit. Cloning process has been verified and expression optimization is up now but I'm...

04 August 2019 7,604 2 View

How can I determine the intact bacterial cell in flow cytometry?

Hi guys! I'm working on a whole cell SELEX project of bacteria for developing a diagnostic tool. Most of bacteria are less than 2 micrometer in size that is interfered with many noises . Please...

12 March 2018 8,339 5 View

Why FITC-nucleotide conjugate emission vanish during SELELX process?

Hi there! I'm using FITC-primer conjugate for amplification of DNA library. The florescent tag is necessary for investigation of SELEX proceeding by flow cytometry technique. The problem is...

04 February 2018 5,830 8 View

How can I optimize a sandiwich aptamer-antibody system?

Hi there!! I have design an aptamer-antibody sandwich ELASA system for a whole cell detection test. Can I follow experiment design process like taguchi designs or should I consider Chequerboard...

04 September 2017 3,173 2 View

Is there any avaliable commercialized product of aptameric(ssDNA) diagnostic system?

Hi guys!! Actually it's really difficult to find the exact information from market!! Could u answer my question on commercialized aptamer based biosensor or any other diagnostic system. I m...

24 August 2017 8,844 1 View

How can I run PAGE for alkaline peptides?

Hi guys I'm runnig poly acrylamid gel electrophoresis for verification of moleular weight of my peptide but it doesnt pepnetrat in gel by runnig SDS-tris-PAGE. Could u plz direct me to a protocol...

11 January 2017 696 2 View

Why is it necessary to optimize ligand and protein structure before docking performance?

Hi every body I'm going to run blind docking for a small molecule with a protein by Autodock 4.2. I have downloaded the pdb files from RCSB . For protein ,I have removed previous bonded ligand...

08 May 2015 9,803 7 View

Why is there a disparity between c-alpha elements and amino acid content?

Hi every body I was going to calculate RMSF for residues of protein after simulation. I chose c-alpha group which contained 667 elements. My protein has 679 amino acids and 50 of them are Gly. So...

24 February 2015 3,234 9 View

How do you set the parameters of a grid file?

Hi everybody, I'm using Autodock4.2 and I have taken these steps respectively. Can anyone assure me that I have been on the right path? I'm concerning about "spacing" and the accuracy of each...

31 January 2015 2,359 9 View

Can anyone help me to resolve the following automated topology builder error for submitted pdb file?

Hi everybody! I'm using ATB to create .itp file for gromacs MD and I have repeatedly received this error message: "the submitted molecule has a critical connectivity missing or contains more than...

11 January 2015 7,627 12 View

Free space on a device for gromacs MD?

Hi I'm working with a restricted free space on a system. How can I aproximate the volume of result for MD? Can I reduce the output on .mdp file if it's needed?

05 January 2015 3,542 3 View