Homa Ahmadi

8 Questions 25 Answers 0 Followers

Questions related from Homa Ahmadi

How cell wall peroperties of l.lactis may change after surface display of a heterogenous antigen?

Hi there! We are applying L.lactis for expression of a heterogeneous antigen using a cell wall anchor subunit. Cloning process has been verified and expression optimization is up now but I'm...

04 August 2019 7,532 2 View

How can I determine the intact bacterial cell in flow cytometry?

Hi guys! I'm working on a whole cell SELEX project of bacteria for developing a diagnostic tool. Most of bacteria are less than 2 micrometer in size that is interfered with many noises . Please...

12 March 2018 8,278 5 View

Why FITC-nucleotide conjugate emission vanish during SELELX process?

Hi there! I'm using FITC-primer conjugate for amplification of DNA library. The florescent tag is necessary for investigation of SELEX proceeding by flow cytometry technique. The problem is...

04 February 2018 5,783 8 View

How can I run PAGE for alkaline peptides?

Hi guys I'm runnig poly acrylamid gel electrophoresis for verification of moleular weight of my peptide but it doesnt pepnetrat in gel by runnig SDS-tris-PAGE. Could u plz direct me to a protocol...

11 January 2017 641 2 View

Why is there a disparity between c-alpha elements and amino acid content?

Hi every body I was going to calculate RMSF for residues of protein after simulation. I chose c-alpha group which contained 667 elements. My protein has 679 amino acids and 50 of them are Gly. So...

24 February 2015 3,190 9 View

How do you set the parameters of a grid file?

Hi everybody, I'm using Autodock4.2 and I have taken these steps respectively. Can anyone assure me that I have been on the right path? I'm concerning about "spacing" and the accuracy of each...

31 January 2015 2,312 9 View

Can anyone help me to resolve the following automated topology builder error for submitted pdb file?

Hi everybody! I'm using ATB to create .itp file for gromacs MD and I have repeatedly received this error message: "the submitted molecule has a critical connectivity missing or contains more than...

11 January 2015 7,562 12 View

Free space on a device for gromacs MD?

Hi I'm working with a restricted free space on a system. How can I aproximate the volume of result for MD? Can I reduce the output on .mdp file if it's needed?

05 January 2015 3,493 3 View