Holly Bamber

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Questions related from Holly Bamber

SDS-PAGE band Identification: Purified human IgG yields multiple bands under reducing conditions?

Purified preps of IgG (via Protein G column) have yielded multiple bands under reducing conditions (mercaptoethanol, 5 mins, 95c) on SDS PAGE. Could you please help me identify the bands by...

05 November 2022 3,227 3 View

What is the role of an amber suppressor for antibody phage display using 12M phage libraries?

I am wholly new to phage display and biopanning. I am looking for a very straightforward explanation as to why suppression of termination of translation is important for phage display.

23 November 2020 8,244 3 View