Hm Choi

3 Questions 2 Answers 0 Followers

Questions related from Hm Choi

Antibody, beads, and control for MeDIP?

Hi, I'm planning to do MeDIP-PCR or qPCR, and it's first try in my lab so I need some help. 1) Most of the papers and protocols I found are using magnetic bead like Dynabead for...

02 March 2022 1,328 0 View

What should I change during bisulfite conversion?

Hi, I'm doing bisulfite sequencing to study DNA methylation status of my target gene. I'm using QIAamp DNA Mini kit(QIAGEN) for obtaining gDNA samples and EZ DNA Methylation-Lightning Kit(Zymo...

17 December 2021 8,831 2 View

How much colonies do I have to select for bisulfite sequencing?

Hi, I'm starting to do bisulfite sequencing, and I'm the first person to do this experiment in my lab so I need some advices. From numerous protocols and papers, when doing cloning-based bisulfite...

07 October 2021 6,719 2 View