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Dear All, I am facing problem with commonly used expression strain E.coli BL21DE3. With transformed plate, I picked up single colony and inoculated in 10mL LB media (with Kanamycin 30ug/ml). Next...
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Hello All, I would like to know how can I resolve my 2 proteins which is 23.5 kDa and 25 kDa sized. I tried 14% hand-casted, various pre-casted gels. Using 14% I could achieve only slight...
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