14 Questions 31 Answers 0 Followers
Questions related from Himanshu Sharma
Hi Folks, I want to detect biotinylated oligos using non-radioactive methods and one of such primary methods is using a Streptavidin-AP conjugate followed by the normal detection methods using...
03 January 2022 1,945 3 View
Are there any ethical issues associated with using C.elegans for infection-related research studies? Any references will be highly appreciated.
23 August 2020 2,903 1 View
I am trying to understand if, two different ways of performing enzyme binding kinetics may impact the final Kd values and if yes to what extent for slow and fast reactions. For more context...
18 March 2019 4,198 3 View
I need to compare level of expression of a protein in E.coli cell lysates. In order to do so we want of take equal amount of protein from cell lysates. But I have too many samples to be processed...
05 March 2018 7,823 4 View
Micrococcal nuclease(MNase), is a nuclease of choice for performing bacterial ribosome footprints. The literature vouches for MNase to be efficient with low cleavage of rRNA. During my use of the...
16 April 2017 9,366 0 View
I use freeze thaw lysis method for lysis of E.coli and use it for isolating ribosomes. Although the cell lysis happens and i get respectable yields, still I feel the cells lysis is not happening...
10 April 2017 5,601 6 View
I am using IgY fractions for detecting bacterial proteins. The antibodies are verified to detect the purified protein of interest. When i use the antibodies in combination with cell lysate,...
04 April 2017 1,871 7 View
Has anyone done dideoxy sequencing using fluorescent probes? I am trying to optimize a method to sequence a pure RNA sample. I have been trying to perform the reverse transcription based dideoxy...
04 June 2016 5,663 2 View
I am trying to optimize a primers extension assay with 2-3 kb RNA fragments to study RNA modifications (methylation and pseudouridylation). I was wondering if anyone has an idea about the minimum...
02 April 2016 3,952 2 View
Recently i came across E. coli RRI strain, its being usually used for plasmid preps.It has carries a recA gene that has been introduced in the parent strain to create RRI. I was suspecting there...
27 May 2015 5,006 4 View
I have just started to perform ITC experiments but every time I am facing a DP value error. The question might be very basic as I have just started to use the ITC200 so please bear with me on...
28 July 2014 4,115 11 View
I am planning to carry out MS for a protein sample isolated after a pull down experiment with bacterial cell lysate. I expect to get a complex of proteins against the bait protein, which can be...
15 June 2014 8,810 1 View
I have been trying to perform an enzymatic biotinylation reaction followed by capturing the biotiylated proteins with streptaidin sepharose beads form GE Helathcare.To do so, I want to remove the...
22 April 2014 2,628 3 View
I am trying to find a method to observe RNA binding for an alkaline protein (pI 9.2), but if I run the protein-RNA complex in a conventional native-TBE PAGE(4-6%), I find the protein stuck onto...
18 January 2014 4,159 4 View
