8 Questions 18 Answers 0 Followers
Questions related from Himanshu Sharma
Are there any ethical issues associated with using C.elegans for infection-related research studies? Any references will be highly appreciated.
23 August 2020 2,786 1 View
I am trying to understand if, two different ways of performing enzyme binding kinetics may impact the final Kd values and if yes to what extent for slow and fast reactions. For more context...
18 March 2019 4,013 3 View
I need to compare level of expression of a protein in E.coli cell lysates. In order to do so we want of take equal amount of protein from cell lysates. But I have too many samples to be processed...
05 March 2018 7,760 4 View
I use freeze thaw lysis method for lysis of E.coli and use it for isolating ribosomes. Although the cell lysis happens and i get respectable yields, still I feel the cells lysis is not happening...
10 April 2017 5,538 6 View
I am using IgY fractions for detecting bacterial proteins. The antibodies are verified to detect the purified protein of interest. When i use the antibodies in combination with cell lysate,...
04 April 2017 1,815 7 View
I am trying to optimize a primers extension assay with 2-3 kb RNA fragments to study RNA modifications (methylation and pseudouridylation). I was wondering if anyone has an idea about the minimum...
02 April 2016 3,912 2 View
Recently i came across E. coli RRI strain, its being usually used for plasmid preps.It has carries a recA gene that has been introduced in the parent strain to create RRI. I was suspecting there...
27 May 2015 4,933 4 View
I am planning to carry out MS for a protein sample isolated after a pull down experiment with bacterial cell lysate. I expect to get a complex of proteins against the bait protein, which can be...
15 June 2014 8,760 1 View
