Himanshu ...

6 Questions 3 Answers 0 Followers

Questions related from Himanshu ...

Why LM2 cell line after transfection not giving different peak during FACS sorting?

I am working with LM2 cells (a metastatic derivative of MDA-MB-231) that already have some baseline fluorescence, and I transfected them with GFP. after transfection i can see clear GFP expression...

02 July 2025 2,000 3 View

How to do the transfection by lipofectamine method? at what ration we will add lipo:dna?

lipofectamine method for the transfection in MDA-MB231 cell line

19 October 2023 3,376 0 View

How to design primer for transgenic mice after knockout of particular gene?

i want to see whether particular gene is knockout or not from the mice? how to synthesize the primer for the same?

04 March 2022 5,968 1 View

Western blotting for animal tissue extract?

I am doing western of mice organ extracts. I am using 3% BSA and overnight blocking followed by 6 hour primary antibody incubation at 4 degree and then 2 hour secondary antibody incubation. The...

04 March 2022 364 2 View

Age related study in mice from month to year related senescence

i want to see the age related senescence . which experiments or new technique to show the same other than IHC and western?

01 January 1970 7,685 2 View

Pharmacokinetics- drug cross blood brain barrier or not. how detect

we want to test a new drug whether it cross the blood brain barrier or not and useful to treat the brain cancer i.e. glioma. how can we detect whether the compound crossing the blood brain barrier...

01 January 1970 1,964 1 View