Why am I unable to generate a knock-out IPSC line using CRISPR Transfection by lipofectamine and electroporation?

My IPSC clones (not estbished lines) are fargile that when i transfect them with Cas9 palsmids - the cells die. I have used lipofectamine, and electroporation method for transfection. But cells...

12 June 2023 9,770 3 View