Hiba Kady

4 Questions 1 Answers 0 Followers

Questions related from Hiba Kady

When using PCR-RFLP to detect a SNP ina promoter, is it obligatory to use SSCP & 32p labled dNTP?

Hey all, I am about to do a research the requires investigating a SNP in the promoter of the bradykinin 1 receptor B1R (G-699 to C) substitution. The paper I found uses SSCP and [32P] - alpha...

07 July 2019 5,570 1 View

Is there a RFLP technique that is not PCR-based?

Usualy the restriction fragment length polymorphisms technique is referred to by PCR-RFLP which means you should do a PCR reaction first. Is there any non-PCR based RFLP technique?

08 August 2016 8,635 3 View

Is there a difference in using TBE or TAE buffers for running a gel when it comes to the length of the amplicon?

Is it wrong to use TBE buffer when running genomic DNA? and why?What are the main differences between those two buffers?

08 August 2016 9,896 3 View

Could you please provide me with examples of diseases caused by multiple nucleotide polymorphisms (i.e. DNPs and TNPs)?

xNPs and diseases

08 August 2016 1,765 0 View