Amicon ultra-10kDa: How can I concentrate cell culture supernatant to measure a secreted protein in WB?

Hi, deal all. We are trying to detect a secreted protein, about 20-35kDa, in the cell culture. What we used was the Amicom ultra-10kDa. We rinsed the filter with ddH20, centrifuged cuture...

28 April 2023 6,327 6 View