Henry Wong

11 Questions 3 Answers 0 Followers

Questions related from Henry Wong

Why do I get my untreated pBR322 bands at between 2500-3000 bp but not 4000 bp?

I've conducted a quality check of my pBR322 using agarose gel electrophoresis. I got my supercoiled form plasmid band at around 2500-3000 bp and open circular form band at around 6000 bp. Why...

22 December 2016 9,277 3 View

Why do I get my untreated pBR322 bands at between 2500-3000 bp but not 4000 bp?

I've conducted a quality check of my pBR322 using agarose gel electrophoresis. I got my supercoiled form plasmid band at around 2500-3000 bp and open circular form band at around 6000 bp. Why...

12 December 2016 9,836 5 View

Will the concentration of SDS and Proteinase K affect the yield of DNA in PCI method of DNA extraction?

I'm using PCI method for genomic DNA extraction from E. coli. I wonder will the concentration of SDS and Proteinase K in my lysis buffer be a consideration for a good yield of DNA? Thank you for...

11 November 2016 651 6 View

Oxidised phenol for phenol:chloroform:isoamyl alcohol method of DNA extraction?

I'm gonna carry out genomic DNA extraction from E. coli using PCI method. Is it still acceptable to use PCI with its bottom layer that has already turned slightly orange in colour? Will I still...

11 November 2016 6,038 3 View

Is there any other method that is able to provide yield as good as phenol:chloroform extraction method for genomic DNA isolation from E. coli?

I'm looking for an alternative method for the extraction of genomic DNA from E. coli. Is there a simpler way other than using phenol:chloroform and extraction kit? I've found a method that was...

11 November 2016 9,448 4 View

Bacteria titre for genomic DNA extraction using phenol: chloroform extraction method?

I'm trying to extract genomic DNA from E. coli using phenol:chloroform extraction method (as suggested by http://www.bio-protocol.org/e97). How do I ensure that the overnight bacteria culture...

25 October 2016 7,765 3 View

Rationale of using pBR322 in gel retardation assay?

I'm currently studying the interaction of a synthetic antimicrobial peptide with DNA using gel retardation assay. I was given pBR322 as the study material for DNA. I'm currently looking for the...

15 October 2016 2,951 0 View

Concentration of plasmid pBR322 to be used for DNA-antibacterial peptide interaction study?

I've been given pBR322 to study the DNA binding property of a synthetic peptide. How do I determine the concentration of plasmid for my absorption spectroscopy assay? Thank you for pondering on...

10 October 2016 9,895 2 View

How to determine titre of increasing peptide concentration in gel retardation assay?

I'm studying the interaction between DNA (pBR322 plasmid) and a synthetic antibacterial peptide using gel retardation assay/electrophoretic mobility shift assay (EMSA). The plasmid is set fixed at...

10 October 2016 547 1 View

Rationale of using pBR322 in gel retardation assay?

I'm currently studying the interaction of a synthetic antimicrobial peptide with DNA using gel retardation assay. I was given pBR322 as the study material for DNA. I'm currently looking for the...

10 October 2016 6,075 0 View

Hi all. Is there a way to conduct gel retardation assay without manually isolating plasmids from a bacteria?

The study is currently working on the binding properties of antibacterial peptide to plasmid DNA of Pseudomonas aeruginosa after the discovery that this particular ABP (known to have membrane...

08 August 2016 9,222 1 View