Hendrik Gremmels

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Questions related from Hendrik Gremmels

Can you combine SYBR Green and Taqman in one reaction?

The above seems like a very unnatural thing to do, so let me elaborate a little more on my research question. I want to develop a PCR reaction that serves both to detect a pathogen and also gives...

07 July 2018 4,454 2 View

Synergistic vs additive stimuli if one stimulus is binary?

A quick question about scientific semantics regarding interaction of stimuli: I have two stimuli, A and B that induce quantifiable effect E. A is a drug and shows a dose-response curve, B is a...

07 July 2015 5,230 6 View

Measuring telomere length in leukocyte (sub)-populations via Flow-FISH?

I was hoping that some of you might be able to help me set up an experiment in which I want to measure telomere length of MNCs (of a certain patient population) by Flow-FISH. As I'm completely new...

07 July 2013 1,548 1 View

Hierarchical/mixed models for the analysis of cell culture experiments - when is it pseudoreplication?

In this question/thread I'd like to hear your opinions about various designs of typical cell culture experiments. It's a complex matter and I have several questions but I'll try to squeeze most of...

06 June 2013 4,479 5 View

What is the easiest way to measure (fractional) receptor occupancy of a tyrosine kinase receptor?

I would like to quantify the fraction of occupied receptors of a growth factor receptor at various concentrations of ligand and correlate it to a biological process. How do you go about this? My...

11 November 2012 5,159 3 View