Helder Anderson Pinto Da Silva

4 Questions 3 Answers 0 Followers

Questions related from Helder Anderson Pinto Da Silva

Promoter cloning in the pDONR P4-P1,r Entry Vector: An AttB4 primer lacking one G in the 5-prime ... It's a problem for BP clonase ?

Hy everyone, I have been working hard to clone an Arabidospis promoter in the pDONR p4-p1,r Entry Vector, but it didn't work until now !!! When checking my primers, I obseved that Attb4 primer...

12 December 2017 9,401 0 View

Promoter cloning in the pDONR P4-P1,r Entry Vector: An AttB4 primer lacking one G in the 5-prime ... It's a problem for BP clonase ?

Hy everyone, I have been working hard to clone an Arabidospis promoter in the pDONR p4-p1,r Entry Vector, but it didn't work until now !!! When checking my primers, I obseved that Attb4 primer...

12 December 2017 8,560 1 View

How can I perform an Agroinfiltration protocol in Nicotiana benthamiana plants using the proteossoma inhibitor MG132?

Hello everyone, I need a protocol for Agroinfiltration in Nicotiana benthamiana plants by using the proteossoma inhibitor MG132. I have been searched in the literature and several papers using...

11 November 2016 4,956 4 View

PENTR/D-TOPO Problems: In several clones the vector seems to close without my gene. Is it possible? What can I do to improve my protocol?

I have been trying to clone Sugarcane genes in the pENTR/D-TOPO cloning system. However, for almost of my obtained Kanamicin resistant clones I don't have success in the insert confirmation using...

08 August 2016 2,183 3 View