Heemin Lee

5 Questions 1 Answers 0 Followers

Questions related from Heemin Lee

Why is my SA-β-gal staining not working?

Hello, I performed senescence-associated β-galactosidase (SA-β-gal) staining on my cells, but I am not seeing any staining. What could be the possible reasons? I am inducing senescence in my cells...

18 February 2025 4,680 3 View

How to treat hydrogen peroxide and Cuso4 to each cell?

Hi! I’m planning to induce aging in 3T3-L1 cells by treating them with compounds that cause cellular senescence. After inducing senescence, I want to validate the aging markers I’ve identified....

18 January 2025 5,935 2 View

Is midiprep doesn't work well? or RE digestion doesn't work well?

I want to know what's wrong with my experiment. Why do you think pMD2.G sample cut band appear 3? Thank you for helping

07 August 2024 8,580 0 View

Why colony does'n come up when I do transformation pMD2.g?

I am conducting an experiment using the results from pMD2.G and psPAX midiprep Q prepared by a senior in 2014. The process involves transformation, midiprep, and midiprep. However, despite...

06 July 2024 4,693 1 View

How long is it okay to store liquid incubation product in refrigerator?

Can I store the liquid incubation(after transformation) results in the refrigerator for about a day and use them afterward???

01 January 1970 8,251 2 View