Heba Alaa Eldeen

2 Questions 4 Answers 0 Followers

Questions related from Heba Alaa Eldeen

How I can know the knocking out effect of specific target sequence by the CRISPR/Cas9 using RT-PCR?

I am doing knocking out for specific sequence by pX458 plasmid and I already did qPCR after 72h of transfection, I have used pX458 plasmid without gRNA as negative control but it seems that has...

12 December 2018 8,836 2 View

Does anyone know How I can determine the integrity and stability of Encapsulated Plasmid?

I encapsulated PX330 plasmid inside anionic liposome and I would like to test the integrity and the desired size of this plasmid after encapsulation, I have tried to run sample of this...

01 January 2018 7,279 0 View