Hatty Douthwaite

6 Questions 6 Answers 0 Followers

Questions related from Hatty Douthwaite

Using RNAseq data how can I demonstrate my human macrophages are more like macrophages than other cell types? and compare them to other Mac subsets?

I have RNAseq data from some human WT and KO macrophages. I have a list of DEGs from this comparison. However in addition I have been asked to demonstrate using the data I have that they (both...

15 February 2022 5,917 2 View

Can I get some advice on how & where I can perform bioinformatics analysis on RNA-seq data?

i wondered how others who have used commercial genomics cores for RNAseq then go about performing further bioinformatics analysis (beyond the basic sequencing QC and mapping provided)? More...

15 January 2020 8,732 5 View

HELP - Online genetics tools/ programs help & knowledge please?

I am working with some human WT and receptor KO IPSCs (mono & bi-allelic clones). The knock-outs have been engineered in a core facility by means of single bp deletions. Having expanded &...

24 July 2019 5,316 3 View

MLR protocol and CFSE labelling, any advice on avoiding cell death?

I am trying to perform a modified MLR adding CD14+ monocytes to CD4+ T cells (both positive MACS isolation), under different conditions. However at 5 days, even with HEPES in culture media, the...

23 May 2019 7,800 1 View

Can anyone help with optimum Monocyte culture media & conditions?

I am trying to culture human Monocytes with CD4+T cells for 36hr at 37oC. (following positive magnetic bead isolation of these cell types from PBMC). I am unable to identify the CD14+ monocytes...

10 January 2019 8,407 3 View

Do 48-well transwells/ Thincerts exist? If not when using a 24well cell culture plate (previously used 48) is it ok to simply double #cells/volumes?

I have developed a T cell assay using 48well culture plates. However I now want to develop the assay further by separating cell populations using transwells/thincerts in the same assay. So far I...

31 August 2017 985 2 View