Harsha Somashekar

3 Questions 14 Answers 0 Followers

Questions related from Harsha Somashekar

How to amplify extremely high GC-rich template?

I am cloning a whole cDNA of a particular gene and I have designed forward primer at start of 1st exon. The region downstream of forward primer is extremely high GC rich (around 85-89%). Because...

18 September 2020 6,667 11 View

How to measure the weight of anthers in a single Rice flower?

I want to do quantification of some biomolecules viz, cellulose, callose, lignin etc., at different stages of rice anthers (6 anthers per flower) where the weight of anthers also differs at each...

19 July 2019 349 4 View

What's going wrong with plastic sectioning?

I am doing Aniline blue staining in rice anthers to detect callose signaling. When anthers are squeezed and stained, I could detect the signaling. However, when I do plastic embedding with...

11 April 2019 9,249 15 View