Harold Bell

3 Questions 5 Answers 0 Followers

Questions related from Harold Bell

How long can you keep frozen aliquots of Dexamethasone and Methylprednisolone?

I would like to be able to store single use aliquots of both for use in cell culture, dissolved in DMSO. I would dilute them with media/buffer on the day of use. There is insufficient information...

09 September 2017 6,359 4 View

Does anyone know if rbc lysis buffer will cause activation of PBMCs during isolation?

We would like to examine cytokine release as well as MAPK phosphorylation in PBMCs and I would like to be sure the PBMCs will not be stressed and therefore activated by the RBC lyis step. Thanks...

05 May 2017 2,777 3 View

Hi - I am wondering how many human PMBCs are needed to have sufficient protein to perform a western blot- We plan on measuring phospho-p38?

PBMCs, western blot, p38, phosphorylation, total protein

03 March 2017 3,792 7 View