When making selection media for a transfection, do I put the antibiotic in media with FBS and PenStrep or fresh media (without any FBS and Penstrep)?

I am working with HepG2 cells and transfecting them with a plasmid that upregulates the GABRA1 gene. I need to put them in selection media before I observe the results. HepG2 cells are resistant...

08 July 2021 2,947 2 View