Harnoor Sachar

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Questions related from Harnoor Sachar

When making selection media for a transfection, do I put the antibiotic in media with FBS and PenStrep or fresh media (without any FBS and Penstrep)?

I am working with HepG2 cells and transfecting them with a plasmid that upregulates the GABRA1 gene. I need to put them in selection media before I observe the results. HepG2 cells are resistant...

08 July 2021 2,957 2 View

If I increase the expression of a GABA alpha 1 receptor subunit in liver cells (with GABA receptors), will it increase the amount of receptors?

For my research project, I am planning on upregulating the GABRA1 gene, which codes for the GABA alpha 1 receptor subunit in some GABA receptors. According to previous studies, individuals with...

18 December 2019 7,016 0 View