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Questions related from Harmony Borchardt-Wier
I just realized that our massive multiplex (273 primer pairs) was destroying itself by forming primer heteroduplexes when stored in the refrigerator, since single stranded DNA can anneal even at...
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I'm used to my sequencing results starting with good quality (or having just a few unreadable bases) and then dropping of on the 3' end, but for this batch, I had a lot that started off bad/junk...
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We found out the hard way that RNAlater blocks x-rays, and now we have thousands of 20-40mm long fish that we can't x-ray unless we can leach RNAlater back out of the tissues. But I have no idea...
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My lab just ordered custom barcodes, and I have no idea what concentration to make them up to. Has anyone here figured out what the concentration of their stocks is? Or if not, can somebody please...
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I'm doing DNA extractions from RNAlater-preserved tissue, and I don't understand why I don't have RNA contamination (when I don't use RNAse). Does anyone have gel pictures showing where RNA would...
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I know this is a basic question, but bear with me. I know I need to pH it to 8. My problem is knowing what type of solid EDTA to start with, and how much to use for a specific...
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