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Questions related from Harmony Borchardt-Wier
I just realized that our massive multiplex (273 primer pairs) was destroying itself by forming primer heteroduplexes when stored in the refrigerator, since single stranded DNA can anneal even at...
08 August 2025 2,581 2 View
I'm used to my sequencing results starting with good quality (or having just a few unreadable bases) and then dropping of on the 3' end, but for this batch, I had a lot that started off bad/junk...
10 November 2022 6,968 0 View
We found out the hard way that RNAlater blocks x-rays, and now we have thousands of 20-40mm long fish that we can't x-ray unless we can leach RNAlater back out of the tissues. But I have no idea...
11 August 2022 3,303 3 View
My lab just ordered custom barcodes, and I have no idea what concentration to make them up to. Has anyone here figured out what the concentration of their stocks is? Or if not, can somebody please...
18 December 2019 3,979 0 View
I'm doing DNA extractions from RNAlater-preserved tissue, and I don't understand why I don't have RNA contamination (when I don't use RNAse). Does anyone have gel pictures showing where RNA would...
23 August 2019 9,048 1 View
I know this is a basic question, but bear with me. I know I need to pH it to 8. My problem is knowing what type of solid EDTA to start with, and how much to use for a specific...
01 January 1970 8,679 1 View
