7 Questions 19 Answers 0 Followers
Questions related from Harir Mohamed
Dear all, I am using Mushroom Tyrosinase that i have already order it from Sigma Aldrich ; and it is black because of melanine, does any one knows if the melanine interact with Bradford as I used...
06 June 2016 9,034 4 View
I tried to immobilized tyrosinase onto amberlite and without using Gluteraldyde Gluteraldyhde, and i have got 100 percent of bound (immoblize enzyme), aim the problem is there is no activity and i...
06 June 2016 7,511 9 View
Dear All , I have a problem with a crud extract extracted from actinobacteria strain; this compound has an antimicrobial activity. However, after one month in 4 c° it became inactive, have you any...
05 May 2016 4,560 3 View
Dear All, I have enzymatic crud extract from Actinobacteria , I d like to extract and purify Tyrosinase, and when I use L dopa i found a heigh activity and for 2,6 DMP substrat there were no...
05 May 2016 8,639 2 View
Dear all, I want to precipitate protein from supernatant of bacteria in culture and my question is whenever i do ammonium sulfate fraction i get floating pellet but, not getting settled pellet....
03 March 2016 1,480 7 View
Dear Researchers, I performed Tyrosinase purification from bacteria broth medium , but I have difficulty to purify this enzymes because of the presence of melanin. Does someone have any idea on...
01 January 2016 8,048 2 View
I have already performed molecular identification 16 DNA on my actinobacteria isolate. However, I couldn't identify the species because the blasting result gave me 100 of similarity with more than...
01 January 1970 2,100 8 View
