Harikesh Janmanchi

4 Questions 9 Answers 0 Followers

Questions related from Harikesh Janmanchi

Is Resazurin dye reduction assay a good model of quantitative evaluation of bacteria using absorbance as a mode of detection rather than fluorescence?

I am trying to develop a HTS method to screen compounds for there activity against Mycobacterium smegmatis. I have used Resazurin dye for viability detection. Its a blue dye which turns pink...

07 July 2013 4,988 1 View

Is comparitive whole genome sequencing of sensitive or resistant bacteria helpful in finding the drug target or mechanism of resistance?

I am working finding the drug targets of certain drugs whose MOA (mechanism of action) is not known. Will whole genome sequence comparison of the sensitve vs resistant bacterial strain yeild any...

03 March 2013 5,651 10 View

How to identify protein target of small organic molecules?

hi, I am working on identifying protein targets against small molecules. So far I have come across references using affinity chromatography as the method. But the problem associated is modifying...

08 August 2012 2,400 7 View

MHC regulation in pregnancy

I am of the opinion that the downregulation of MHC during pregnancy is due to the high progesteron level in the mothers body. Also females are more prone to autoimmune disease than men and the...

05 May 2010 7,480 1 View