Harald W Platta

4 Questions 3 Answers 0 Followers

Questions related from Harald W Platta

How can I create a completely deubiquitination-resistant protein?

Several publications indicate that the mutation of Gly75 and Gly76 of ubiquitin to Ala protects ubiquitin-fusion-proteins against deubiquitination. However, in our case, the protein of interest,...

29 October 2014 9,801 2 View

Which Aspartyl-Protease inhibitor can enter yeast cells when added to the growth medium?

We would like to inhibit aspartyl-proteases (like Pep4/PrA) in living S. cerevisiae cells. We know that cysteine/serine-proteases can be inhibited by adding PMSF to the growth medium. Is there...

09 October 2014 8,005 2 View

Are there any specific marker proteins for rough and smooth endoplasmic reticulum analysis?

We are working with Saccharomyces cerevisiae. I would like to know if there are specific marker proteins for the rough as well as for the smooth endoplasmic reticulum. These proteins should only...

30 September 2014 6,163 2 View

Is there an alternative drug to Lat A or Lat B which is less costly?

Inhibition of actin polymerization in S. cerevisiae. We want to study the role of actin in the transport of different cargos in S. cerevisiae. The best known method would be to use Latrunculin A...

25 September 2014 6,354 7 View