Harald Lund

2 Questions 6 Answers 0 Followers

Questions related from Harald Lund

CD3-staining on PFA-perfused and frozen mouse CNS tissue?

Hi! We are struggling to perform something which I had hoped would be quite standard. CD3-staining on mouse spinal cord from EAE mice (should be plenty of T-cells). The tissues were PFA-perfused,...

06 February 2017 4,289 1 View

How many microglial cells do I need to be able to do qPCR?

I am planning to sort out microglial cells from mouse brains after percoll separation either by FACS or by using CD11b-magnetic beads. How many cells can I expect from one mouse and how many...

25 November 2013 5,511 7 View