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Hello. I'm trying to do FRET-based in vitro kinase assay these days with MAPK and MAPK inhibitor. I expected kinase activity to decrease in dose-dependent manner when treated with kinase inhibitor...
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I did affinity chromatography with GFP protein, Histidine tag and Imidazole buffer. I did washing with 20mM Imidazole buffer to eliminate non-specific proteins but found out that it luminesces...
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Hello! I'm studying about RNA editing these days and became curious if there is anything like computer simulation program that gives me editing prediction if I input guideRNA and mutation...
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Hello! I've been studying about ADAR which stands for Adenosine Deaminase Acting on RNA. I heard that ADAR only works on double stranded RNA and changes mutant A to I. But isn't mRNA in our body...
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