Hani Alrefai

2 Questions 2 Answers 0 Followers

Questions related from Hani Alrefai

How to boost glycolysis in T cells?

I have a genetically modified mouse line that is showing a characteristic phenotype including low glycolysis levels of the T cells as shown by the glycolytic stress test. I want to test a...

23 September 2021 2,200 0 View

Can I use magnetic cell separation columns (MACs) on methanol permeabilized fixed cells ?

I have extracted spleenocytes from mouse spleens (big Batch) and expanded them using ConA (72h) followed by IL-2 (72h). Trypaned, Ficolled, then Fixed for 20 mins in 1% PFA. After that, counted,...

13 October 2017 494 2 View