Hanh Nguyen

2 Questions 4 Answers 0 Followers

Questions related from Hanh Nguyen

Is 1500 nucleotides RNA too big to do a RNA -protein binding gel shift?

Hello, It is the first time I do a gel shift for RNA. I feel kind of lost. I need to do several RNA protein binding gel shifts. My purify protein is 10 KDa and my RNAs is 200, 600, 1500, 1600...

06 June 2015 7,700 3 View

Is anyone familiar with designing DNA templates for t7 RNA polymerase?

Hi, It is the first time I am working with small RNA-Protein interaction. I need to design DNA template for T7 RNA polymerase to synthesize my small RNA. I just want to make sure I need to put the...

04 April 2015 6,138 6 View