Hang Pham

3 Questions 4 Answers 0 Followers

Questions related from Hang Pham

How to reduce large gap between R work and R free ?

Hi, I am doing refinement for my structure. The resolution is around 2.5A The model building is fitting well in the electron density. I already try more refinement cycle, added water molecules and...

03 May 2019 9,139 1 View

Could you make crystallography plates with stored protein at -80C ?

Since it takes me a week to express and purify protein everytime when I need protein for crystallogenesis experiment, I am wondering if we could store the purified protein at -80C at use it later...

03 August 2016 9,020 1 View

Do you know how to prevent the precipitation of protein after concentrating using Amicon ?

I purified a lipase by HiTrap FPLC and dialyzed the collected samples overnight in a solution of Na2HPO4 (50mM), NaCl (200mM), EDTA (50mM), DTT (2mM), pH 8. Since I got a high volume of sample...

25 July 2016 3,844 12 View