Han Min Ohn

5 Questions 11 Answers 0 Followers

Questions related from Han Min Ohn

Hello, have anyone tried sterilized bacteria from biofilm using UV from biosafety cabinet?

I have read a paper that stated that after 30 minutes sterilization of 96 well microtitre plate with UV from biosafety cabinet, no cfu was recovered from biofilm of E.coli. However, I sterilized...

27 December 2018 7,695 4 View

Quantifying total biofilm masses when result is above readable point of plate reader?

Hi I am using crystal violet assay in microtitre plate for quantifying total biofilm masses formed under different condition and comparing the result. If my OD values are above 3 which is the...

01 August 2018 2,896 2 View

Hi I would like to determine total carbohydrtes from bacterial biofilm polysaccharide. Any one has suitable Dubois protocol?

How many mg of CHO present in 1mg of lyophilized biofilm polysaccharide? I know it varies with bacteria but any one know range?

12 October 2017 917 3 View

Why no EPS Precipitates seen after precipitation with 95% ethanol and centrifugation?

Are EPS precipitated by 95% ethanol at 4'C visible to the naked eye. I am precipitating with ethanol in 15/50ml tubes for 24 hours at 4'C and then transfer the solution to 2ml eppendorf tubes and...

26 September 2017 5,451 0 View

I would like to purify bacterial exopolysaccharide using 26/60 S-200 gel filtration column using an AKTA FPLC system and would like to know details.

I would also like to know the settings for FPLC system such as column flow rate, temperature, buffer to use and would like to know the standards which I have to use to develop molecular weight vs...

01 January 1970 748 9 View