Haley Hallowell

6 Questions 4 Answers 0 Followers

Questions related from Haley Hallowell

Are these lysogenic plaques?

Hello, I'm working with lambda phage, and have worked with other temperate phage in the past. I'm currently trying to definitively determine if the large plaques seen on the picture attached are...

18 November 2020 3,701 4 View

Best antibiotics to deplete specific communities in the murine gut?

Hi everyone, What antibiotics would be best to deplete only Gram+ from the murine gut? And which would be best to deplete only Gram-? I was originally going to use Ampicillin and Vancomycin, as...

11 July 2017 3,152 2 View

Sequencing Fungal portion of microbiota?

Hey all, Can anyone tell me why it is preferred to use sequencing of the ITS region of the 18s in fungi compared to shotgun sequencing? I would assume that shotgun would be preferred, based on...

10 April 2017 2,222 2 View

What is the purpose of a Permanova test, specifically in terms of the gut microbiota?

I am currently investigating Archaeal and Fungal populations in a micro biome dataset, and while digging in the literature I came across the linked paper below in which they utilized Permanovas to...

14 March 2017 1,182 10 View

Has anyone used an ELISA for porcine Calprotectin?

I'm looking to measure calprotectin levels in porcine feces, has anyone worked with ELISA kits that fit this? Any advice would be appreciated, thanks!

26 August 2016 4,733 1 View

Isolating dendritic cells from the murine colon?

I'm currently working on isolation of dendritic cells from the murine colon, but haven't been able to get too much. I'm currently using EDTA washes followed by a collagenase/DNAse digestion, then...

30 May 2016 3,784 5 View