Haider Cuello

3 Questions 7 Answers 0 Followers

Questions related from Haider Cuello

What happens in multiplex PCR (extra long DNA amplification)?

I am performing a multiplex PCR with a total of 19 oligos (forward and reverse), I have tried to amplify this using different annealing temperatures, the reagents I am using are designed for...

20 April 2025 2,141 8 View

Why when I add dntps in my buffer solution of Hydroxy Naphthol Blue with Mg2+ change from purple to dark blue?, is there a solution for this problem?

I am performing a Lamp PCR protocole using Hydroxy Naphthol Blue, I am using 200uM, I found that the Mg2+ suitable for this protocolo is 6mM, however, every time that I add the dntps in the...

16 December 2024 4,986 2 View

Methylation, the best selection to get the real result when is reading the percentage of methylation, a colleague could help me with this question?

I have a important question to analyze the result of methylation percentage, but when we analyze the methylation result we focus in one DNA strand out of the two strands it has, however, we want...

20 April 2024 6,284 3 View