Hadi Ostadi

5 Questions 11 Answers 0 Followers

Questions related from Hadi Ostadi

Could anyone help me regarding increasing stability of harvested mAb supernatante after clarification and sterile filtration?

I am currently working on ocrelizumab purification process. After harvest step of cell culture, cell and other debris was removed by depth filters and sterilized by 0.22 micrometer filter. It was...

21 September 2020 9,271 0 View

How can separate single domain camelid antibody (VHH), from FSH?

I'm currently working on purification of FSH. supernatant was loaded through the capture select FSH affinity resin. Its novel affinity ligand recognize the intact FSH molecule without binding its...

15 April 2019 9,276 5 View

Capture human EPO to the special IMAC resin?

I'm currently working on the purification of EPO which is secreted from CHO cell . for final step of purification I have to applied IMAC-Fe(III) for an especial goal.(Endotoxin removal) could any...

20 April 2017 4,467 10 View

Endotoxin removal, could any one help me regarding this request?

I'm currently working on purification of protein process. In final step of the purification I found out endotoxin in the product, to eliminate it I use ultrafiltration by use of cut off 10 and 30...

23 February 2017 4,037 3 View

Cleavage of fusion protein?

I am currently working on purification protein process. For cleavage of target protein from sumo I applied sumoprotease. the activity of enzyme was too low, could any one recommend me an especial...

13 February 2017 8,416 3 View