Hadeel Al Sadoun

2 Questions 5 Answers 0 Followers

Questions related from Hadeel Al Sadoun

Is there any publication that support the use of CD14 as differentiation marker of murine macrophage?

It is well known that CD14 used to differentiate human monocytes from macrophages. But mouse macrophages may not follow the same expression. So I was wondering, could we use CD14 as marker of...

20 August 2015 6,749 3 View

Does anyone know a gentle way to detach a macrophage differentiated THP-1 without affecting their viability ?

I am working with the THP-1 cell line, and they were differentiated into macrophage-like cells using 5ng/ml PMA. When I try to detach them using accutase (20min-37C), they are successfully...

17 October 2013 7,274 7 View