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Hi everyone I have performed several flow cytometry experiments and I keep getting a basal FITC signal (of unstained cells) higher than FITC isotype control signal (of cells stained with the...
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Hi, I reached to the point I had spare cell-cultures, so I kept some of the cell plates in -80°C (no medium of-course, after cold PBS washing - I removed PBS to be left only with cells). Now I...
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Has anyone ever tried to stain 2 different proteins in a single sample, while they both originate from he same species (1 of them conjugated though)? I have 1 primary antibody (host: mouse)...
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