Hadas Patya

3 Questions 3 Answers 0 Followers

Questions related from Hadas Patya

How come the isotype signal is lower than the basal signal when analyzing flow cytometry?

Hi everyone I have performed several flow cytometry experiments and I keep getting a basal FITC signal (of unstained cells) higher than FITC isotype control signal (of cells stained with the...

27 July 2020 8,502 3 View

Can cells kept in -80°C be used for western blotting?

Hi, I reached to the point I had spare cell-cultures, so I kept some of the cell plates in -80°C (no medium of-course, after cold PBS washing - I removed PBS to be left only with cells). Now I...

26 June 2019 574 2 View

Immunofluorescence - can I use a conjugated antibody and a primary not-conjugated different antibody - both from the same host species?

Has anyone ever tried to stain 2 different proteins in a single sample, while they both originate from he same species (1 of them conjugated though)? I have 1 primary antibody (host: mouse)...

07 May 2019 6,601 6 View