Gustavo Monasterio

4 Questions 6 Answers 0 Followers

Questions related from Gustavo Monasterio

Good protocol for t cell isolation from mouse/rat palatal-gingival tissues?

Hi everyone, I'm trying to obtain T cells from palatal and maxillary gingival tissues of mice and rats to flow cytometry analysis. If anyone has a good protocol, I would be very grateful. kind...

08 June 2017 5,367 3 View

How can I process BALB/c mouse tooth samples to see hard tissues (tooth and bone) in SEM?

I need to take some photos of the alveolar bone destruction during periodontitis of BALB/c mouse samples in SEM, but I haven't found a protocol that mentions how to process the sample to clean it...

21 November 2016 7,562 3 View

Best way to analize and charting qPCR data using deltaRn?

I need to know the form to analize and make charts of relative quantification of differents cytokines mRNA expression, but using the deltaRn, not the Ct provide by the software. I'm using Applied...

12 October 2016 2,309 4 View

Has anyone worked in RNA isolation of mouse (BALB/c) palate mucosa? Any special tip to obtain more RNA??

Now i´m working in RNA isolation of the half part or all the maxilar palate mucosa of BALB/c mouse, with the aim to measure the gene expression of different cytokines with RT-qPCR. But my problem...

11 October 2016 6,370 5 View