Gurusamy K.Muniasamy

6 Questions 10 Answers 0 Followers

Questions related from Gurusamy K.Muniasamy

Is the Topology of a phylogenetic tree same from different tools?

I am very new to bioinformatics. Here,i am trying to make a phylogeny reconstruction of protein sequences following methods like Maximum likelihood (MEGA 6.0 and GARLI) and Bayesian inference...

11 November 2015 4,163 15 View

Any advice on genome-wide screening of homologs?

I have been working with a bacterial protein of gram negative bacteria. At present, i am in search of its homologs in the closely related and, even in lower eukaryte organisms. So i decided to to...

09 September 2015 8,436 3 View

Does anyone have experience in isolating cell organelles from yeast S. cerevisiae using glass bead beater?

Many use enzymes like lyticase to get spheroplasts and subsequently, perform mechanical disruption to disrupt the yeasts cells. The isolation is usually followed by the centrifugation. Apparently,...

12 December 2014 1,768 2 View

What is the best way to study the structure of different proteins - Bioinformatics?

Hi Folks! I am very new to the bioinformatics. I did a fusion protein expression in S. cerevisiae. I am interested to analyse the structure of the fusion protein with the native...

08 August 2014 2,628 6 View

Can anyone help with membrane reconstitution of enzymes?

I would like to know some probabilities in working with the enzymes in the membranous sources such as liposomes. I am working with a bacterial enzyme which is inactive in its monomeric state. It...

06 June 2014 5,126 4 View

Do oligomers and polymers have any effect on their properties in case of DSC and TGA analysis?

I am a beginner in the field of polymeric chemistry and interested to learn about the characteristics of the oligomers and polymers of hydrocarbons espiecally polylactic acid, polysuccinic acid.

07 July 2013 8,887 1 View