Gurjeet Kaur

3 Questions 4 Answers 0 Followers

Questions related from Gurjeet Kaur

Is it essential to characterise the sequence of novel microRNAs after bioinformatic analysis with mirdeep2 through experimental cloning strategy?

I need to experimentally validate the novel microRNAs in my patient cohorts. Before that is it necessary to do cloning of mature/precursor mir sequence followed by sanger. If so , Can anyone share...

04 October 2017 1,939 4 View

What should be the randfold value for predicting novel MicroRNAs ?

I am facing problem in selecting novel microRNAs from small RNA sequencing. mirdeep 2 software was used for prediction of novel microRNAs. Is it necessary to have high mirdeep2 score with high...

29 September 2017 8,674 3 View

Whats the exact function of sequence tagged sites in a gene ? Does the single nucleotide variations affect its function ?

I found single nucleotide change in sequence tagged site . though that region is not part of CDS but its the part of STS.

16 April 2017 1,343 2 View