Gunaseelan Sathaiah

4 Questions 3 Answers 0 Followers

Questions related from Gunaseelan Sathaiah

For screening of drugs against COVID-19 by molecular docking, which is the best target site or protein for COVID-19?

Since there is confusion in selecting a target site with correct PDB ID for molecular docking of drugs. E.g. 3-CL protease has lot of PDB ID, Which one to select?

25 April 2020 3,584 3 View

What all temperatures should I set to perform Gradient PCR with different melting temperatures of forward and reverse primers?

Gene with FP having Tm= 63.5 and RP Tm= 64.5. So what all temperatures should I set for Gradient PCR??

30 March 2018 2,041 3 View

How to perform silver nitrate test for arsenite (III) oxidizing bacteria? Or is there any other method for screening bacterial arsenite oxidization?

My bacterial isolates are capable to grow on 600 ppm concentration of arsenite (III) which is used as a electron acceptor by bacteria. For the confirmation of transforming ability of arsenite by...

21 December 2017 8,932 1 View

How to dissolve BTEX (Benzene Toluene Ethylbenzene Xylene) in minimal media for aromatic hydrocarbon degradation assay?

I am performing hydrocarbon degradation assay (diphenyl carbazide assay) using bacteria. So I need to dissolve non polar (hydrocarbons) such as Benzene Toluene Ethylbenzene and Xylene in minimal...

21 December 2017 5,823 0 View