Guilherme Souza

2 Questions 6 Answers 0 Followers

Questions related from Guilherme Souza

Trouble with Absorption measurements?

I'm doing a direct enzymatic assay, measuring the formation of a product at 240nm. However, as a control, I started taking off some components of the reaction, and found that even when there were...

02 February 2017 2,574 14 View

Help with coupled enzymatic assay?

I am trying to re-determine the Km of the enzyme I'm working with. For some reasons, I'm trying to do this using a coupled assay. I am having trouble with that, because the Km that I am finding is...

01 January 2017 8,096 4 View