Grace Lachica

2 Questions 4 Answers 0 Followers

Questions related from Grace Lachica

Early amplification in both NTC and NEC in qPCR (using taqman probes)?

Hi! I've been trying to decipher what happened to some of our runs with early amplification in the NTC and NEC. This has happened to us twice now. For background, we are using a mastermix of PCR...

10 September 2020 4,751 5 View

Why am I getting a different product amplified when doing hot start?

Hi! I'm doing a study of garlic SSRs. I'm trying different published SSR markers for garlic and currently optimizing the PCR conditions. I have tried traditional PCR, touch down (TD) and hot start...

04 August 2014 9,348 8 View