Grace Lachica

5 Questions 14 Answers 0 Followers

Questions related from Grace Lachica

Early amplification in both NTC and NEC in qPCR (using taqman probes)?

Hi! I've been trying to decipher what happened to some of our runs with early amplification in the NTC and NEC. This has happened to us twice now. For background, we are using a mastermix of PCR...

10 September 2020 4,952 5 View

How can I improve transformation?

I need to do transformation for subcloning as well as expression of my GOI. I have successfully ligated the gene (PCR product) into the pET vector as confirmed by PCR. My inserts are the sizes of...

23 October 2015 9,583 20 View

Anyone knows a good protocol for extracting DNA from garlic leaf?

I have to extract DNA from a large number of garlic leaf samples for SSR analysis. I am doing a bead milling technique using SPEX SamplePrep GenoGrinder. My current protocol involves using...

15 October 2015 9,774 9 View

How can I design EST-SSR marker for garlic?

I am currently working on designing SSR primers for garlic and I'm planning to use EST sequences. I have downloaded ESTs from NCBI's GenBank (though, been trying the garlicdb website but to no...

18 February 2015 6,675 8 View

Why am I getting a different product amplified when doing hot start?

Hi! I'm doing a study of garlic SSRs. I'm trying different published SSR markers for garlic and currently optimizing the PCR conditions. I have tried traditional PCR, touch down (TD) and hot start...

04 August 2014 9,399 8 View