Gladys Crews

2 Questions 6 Answers 0 Followers

Questions related from Gladys Crews

Does anyone know of any real time PCR primers that detect E6 and E7 in HPV positive and negative cells?

I have been trying to detect E6 and E7 in cell lines that are suppose to be either HPV negative or positive based on information (background) that the lines have varied percentages of HPV DNA. If...

12 December 2016 5,037 3 View

Does anyone have a good MTT assay protocol for adherent cells?

I've been working with some adherent cells (cancer cells) and trying to use an MTT assay to evaluate % survival 3 days after transfection with lipofectamine and miRNA mimics. I'm plating cells in...

11 November 2014 8,309 7 View