Why does C4-2B cell line stop to proliferate after a month in medium (T-medium) containing 10% of charcoal-stripped serum?

I have high passage C4-2B (20) that grow very well in medium with FBS, but not in medium with 10% of charcoal stripped serum. This line should be castration resistant, could the passage number...

06 June 2015 3,637 1 View