Girishkumar K. Kumaran

3 Questions 20 Answers 0 Followers

Questions related from Girishkumar K. Kumaran

How much time will it take AF488 to lose fluorescence if it is left open in light?

I wish to know if someone has experience with leaving AF488 out in light.

03 March 2018 5,501 4 View

What is the best way to prepare pichia pastoris whole cell lysates for SDS PAGE to check for expression?

I am trying to purify a membrane protein using Pichia pastoris expression system. I wish to know what is the best way to prepare whole cell lysates to check for expression

03 March 2016 7,781 5 View

What is the role of biotin in BMGY media?

I wish to know the role of biotin in BMGY (Buffered Glycerol Complex Medium) media used for Pichia pastoris cultures.

02 February 2016 7,755 6 View