Gil Diamant

4 Questions 14 Answers 0 Followers

Questions related from Gil Diamant

How do I propagating and expend lymphocytes from primary human blood?

We have frozen PBMC isolated from human blood samples - (~3-10 million cells per patient). We want to use them mostly for testing binding of various target molecules by BCRs and TCRs. This means...

01 January 2019 5,136 3 View

How can I sort IgE+ B cells?

I want to stain PBMC such that I will be able to sort B cells that are IgE positive and sequence their genomes. Does anybody of the fine scientists here have experience with this? Particularly,...

03 March 2018 7,481 3 View

What is the best tag to clone unto a protein we will use to mark cells for flow cytometry?

I want to clone a tag onto protein X, a protein which we are expressing and purifying in the lab. We will than incubate protein X with cells to see which cells bind it. I want to stain the sample...

03 March 2018 5,135 7 View

What could be causing low efficiency in previously excellent qPCR reactions?

Greetings all, I've been performing qPCR on an ABI "StepOnePlus" machine for the past year. I produce RNA either by kit or Trizol, perform RT using Quantabio "qScript cDNA synthesis kit", and...

11 November 2016 2,016 10 View