Gian Maria Niccolò Benucci

5 Questions 18 Answers 0 Followers

Questions related from Gian Maria Niccolò Benucci

What is the best technique/kit to extract fungal DNA for genome sequencing purposes?

Sequencing facilities often require a very high quantity and quality of genomic DNA to perform de-novo genome sequencing. Many commercial DNA extraction kits are available in the market and many...

11 November 2015 4,873 1 View

Does anyone have any knowledge on genome size determination in Fungi?

With the rapid growth of whole-genome sequencing in Fungi, I am wondering if an efficient and user-friendly method for estimating genome size already exists. Especially in some range of fungal...

06 June 2014 1,252 3 View

What is the best approach to illumina MiSeq library preparation for microbial communities studies?

We are going to perform a fungal/bacterial community ecology study. We have a set of 24 samples that should be selectively amplified for ITS2 and 16S DNA regions, for a total of 48 different...

12 December 2013 5,912 9 View

Are cut-off values for MOTUs approximation of taxonomical species still a valid compromise in community ecology studies?

How is possible to establish sequence identity cut-off values for species approximation is a global problem for molecular ecologist and taxonomists. In the new molecular era, where high-throughput...

11 November 2012 8,724 2 View

Does anyone have any experience with a DNA extraction kit for soil samples that works for more than 1g of soil?

I need to extract meta-genome from soil samples but I have start from big soil quantity, I will estimate approximately the whole living community.

10 October 2012 3,563 24 View