Ghulam Abbas

5 Questions 7 Answers 0 Followers

Questions related from Ghulam Abbas

What is the optimum time to harvest Lentivirus from HEK293T cells after transfection?

I have tried till now 72 hours harvesting but I'm not sure which time would be the optimum for maximum potency? Can somebody please guide me in this regard?

11 November 2019 3,145 3 View

What is the best concentration of Polybrene during lentivirus infection in AML cells?

I have tried different concentrations of polybrene during experiment but as AML cells are difficult to transfect so could not determine which is the best concentration? To what extent it causes...

11 November 2019 5,939 2 View

What are the suggested methods to improve the plasmid transfection efficiency in AML suspension cell lines?

I gave tried lentiviral infection system but the transfection efficiency is not very good? Kindly suggest me some other methods as well as how can I improve the transfection efficiency in...

06 June 2019 9,767 4 View

What are the effective ways to synchronize cell cycle? Does the serum deprivation will work for cancerous cell lines?

How it will work for adherent cells and cells in suspension? Is there any cells toxicity probability?

01 January 2019 9,883 4 View

How do I prevent HeLa cell lines cell culture plate from contamination?

In my experiment I got contaminated HeLa cell culture plate. I don't know the reason behind it. can anybody please suggest something worthy to resolve such kind of issues?

09 September 2017 7,054 14 View